MtoZ Biolabs utilizes advanced high-resolution mass spectrometry platforms to offer the comprehensive protein sequencing analysis service which enables systematic and comprehensive analysis of protein sequences. This service is widely applied in protein integrity confirmation, sequence variation verification, and mutation site comparison, providing reliable data support for obtaining accurate protein sequence information.
Overview
Proteins are macromolecules formed by amino acids linked in a specific order, and their amino acid sequences determine their structure, function, and stability, making them essential to understanding biological processes. Protein sequence detection can help confirm protein identity, discover sequence differences, verify mutations and evaluate the quality of recombinant proteins by analyzing the sequence of amino acids. It is widely used in recombinant protein development, structural research and biological sample quality assessment, providing key data support for related research.
Comprehensive Protein Sequencing Analysis Service at MtoZ Biolabs
MtoZ Biolabs provides protein sequencing services that include, but are not limited to, the following:
1. Antibody Sequencing
Utilizes high-resolution mass spectrometry to analyze heavy and light chain sequences, supporting antibody development, optimization, and consistency verification.
2. Peptide Sequencing
Accurately determines the amino acid order of peptides through mass spectrometry, suitable for quality confirmation of peptide drugs, bioactive peptides, and labeled peptides.
3. De Novo Sequencing
Reconstructs complete amino acid sequences using fragment ion information when no reference sequence is available, ideal for unknown or novel protein studies.
4. Edman Degradation Sequencing
Uses classical chemical methods to sequentially identify N-terminal amino acids, suitable for short peptides and high-purity proteins.
5. Mass Spectrometry Sequencing
Performs rapid and accurate protein sequence identification using high-resolution mass spectrometry, applicable to a wide range of biological samples.
6. N/C-Terminal Sequencing
Precisely characterizes the N-terminal or C-terminal regions of proteins to confirm modifications, truncations, and terminal integrity.
7. Full-Length Protein Sequencing
Systematically resolves complete amino acid sequences, suitable for recombinant protein verification, sequence integrity confirmation, and quality consistency assessment.
Chugunova, A. et al. J Proteome Res. 2018.
Figure1. Protein Sequencing Principles.
Why Choose MtoZ Biolabs?
✅ High-Resolution Detection: Leverages advanced mass spectrometry platforms to achieve precise, sequence-level analysis.
✅ High Coverage Sequencing: Enhances sequence coverage and continuity through combined fragmentation strategies.
✅ High Throughput Capability: Supports simultaneous processing of multiple samples to meet large-scale sequencing needs.
✅ Standardized Analytical Workflow: Ensures consistent, reproducible, and highly reliable results.
✅ Customizable Solutions: Provides flexible sequencing strategies and technical combinations tailored to specific research needs.
Applications of Comprehensive Protein Sequencing Analysis Service
1. Protein Integrity Studies
Used to evaluate whether proteins maintain sequence and structural stability during processing, expression, or storage.
2. Functional Correlation Exploration
Assists in identifying functional regions, active segments, and conserved features of proteins through sequence characteristics.
3. Source Consistency Verification
Suitable for comparing protein consistency across different batches, production platforms, or expression systems.
4. Process Optimization Assessment
Monitors sequence variations to evaluate the impact of expression, purification, or processing steps on protein characteristics, supporting workflow optimization.
5. Evolution and Diversity Studies
Used to compare protein sequence differences among populations or species, supporting evolutionary relationship and diversity analyses.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Protein Sequence Analysis Results
4. Related Mass Spectra and Visualized Charts
5. Raw Data Files
6. Comprehensive Analysis Report
FAQ
Q1: What types of samples are suitable?
A1: The comprehensive protein sequencing analysis service can accept protein samples derived from blood, serum, plasma, cell lysates, tissue extracts, and other biological sources. It is also suitable for recombinant proteins, peptides, and antibodies. Samples should have good solubility and basic purity to ensure accurate sequence analysis. For low-abundance or highly impure samples, pre-processing is recommended to improve sequencing performance.
Q2: What is the service general workflow?
A2:
Q3: What data formats are provided?
A3: MtoZ Biolabs offers multiple standardized output formats, including:
- Raw mass spectrometry data files (such as .raw platform formats)
- Sequence analysis results and variation information (Excel/CSV)
- PDF analysis report (including experimental methods, analytical workflow, and key conclusions)
- Spectral files (such as fragment ion maps and sequence coverage maps, PNG/TIFF)
If special analytical requirements exist, data formats can be customized according to project specifications.
Q4: How should I prepare my samples?
A4: To ensure optimal sequencing results, it is recommended to prepare samples as follows:
(1) Sample purity: Avoid high salt, detergents, and other components that may interfere with digestion or ionization.
(2) Sample storage: Store at 4℃ for short-term use; for long-term storage, keep at -80℃ and avoid repeated freeze-thaw cycles.
(3) Sample transport: Ship with dry ice or ice packs under sealed conditions to prevent leakage or contamination.
(4) Additional information: Provide sample source, concentration, buffer composition, and pre-treatment steps to help determine the most suitable sequencing strategy.
For more information, please refer to Sample Submission Guidelines for Proteomics, Sample Submission Guidelines for Metabolomics.
Start Your Project with MtoZ Biolabs
Contact us to discuss your experimental design or request a quote. Whether you aim to confirm the accuracy of a protein sequence or investigate sequence differences between samples, we can provide you with professional and reliable sequencing support.
