MtoZ Biolabs provides Discovery Proteomics Service to help researchers comprehensively profile protein expression and regulation across cells, tissues, and biofluids using high-resolution LC-MS/MS. Our discovery proteomics solutions support unbiased biomarker screening, disease mechanism studies, and target identification across biomedical, clinical, microbial, and plant research.
What Is Discovery Proteomics?
Discovery proteomics refers to large-scale, hypothesis-generating analysis of the proteome to identify as many proteins and peptides as possible in an unbiased manner. Unlike targeted proteomics, which focuses on a predefined panel of proteins, discovery approaches survey thousands of proteins in parallel to uncover unexpected changes, new pathways, and novel biomarkers.
Mesri, M. Adv Med. 2014.
Figure 1. Comparison Between Targeted Proteomics and Discovery Proteomics
By measuring global protein abundance, post-translational modifications, and pathway-level changes, discovery proteomics enables you to:
🔸Reveal disease-associated protein signatures and molecular mechanisms
🔸Identify candidate biomarkers for diagnosis, prognosis, or treatment response
🔸Prioritize drug targets and pathways for therapeutic intervention
🔸Compare proteome states across conditions, time points, species, or treatments
Because proteins are the direct executors of cellular function, discovery proteomics provides a closer readout of biological activity than DNA or RNA alone. It is a powerful first step to move from broad biological questions to specific, testable targets that can later be validated with targeted or functional assays.
Discovery Proteomics Service at MtoZ Biolabs
MtoZ Biolabs provides Discovery Proteomics Service using label free and DIA based strategies combined with nanoflow LC, trapped ion mobility mass spectrometry, and nanoparticle based enrichment. This integrated platform enables deep, unbiased profiling of complex samples with high sensitivity, resolution, and quantitative robustness.
Our workflow covers end to end analysis, from sample preparation and protein extraction to enzymatic digestion, nanoflow separation, LC MS/MS acquisition, and dedicated bioinformatics. By enriching and retaining low abundance proteins in biofluids with tailored nanoparticle chemistries, we can flexibly scale proteome depth while maintaining quantitative accuracy. The platform supports comprehensive analysis of PTMs and proteoforms, characterization of exosomal and membrane associated proteins, and high confidence identification based on rich peptide fragmentation patterns.
Discovery proteomics solutions at MtoZ Biolabs are applicable to a wide range of biological matrices and species, including preclinical animal models and clinical samples. They are well suited for global protein expression profiling, pathway and network analysis, biomarker hypothesis generation, and early stage target discovery across diverse biomedical and translational research projects.
Workflow of Discovery Proteomics Service
1. Experimental Design and Consulting
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Discuss biological questions, sample types, group structure, and required depth
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Recommend appropriate platforms (for example DIA, 4D-LFQ, label-free) and replication strategy
2. Sample Preparation and Protein Digestion
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Protein extraction from cells, tissues, biofluids, or other matrices
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Protein quantification, reduction, alkylation, and enzymatic digestion
3. LC-MS/MS Analysis
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High-resolution LC-MS/MS using optimized gradients and instrument settings
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Discovery-oriented acquisition (for example DIA or DDA-based global profiling)
4. Data Processing and Quantitative Analysis
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Database search and false-discovery-rate controlled identification
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Protein quantification, normalization, and statistical comparison across groups
5. Biological Interpretation and Reporting
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Pathway, GO, and network enrichment analyses
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Highlighting key differentially expressed proteins and pathways linked to your hypothesis
Why Choose MtoZ Biolabs
✔️Advanced Analysis Platform
MtoZ Biolabs established an advanced discovery proteomics analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.
✔️Customized Project Design
Study designs aligned with your biological questions, sample availability, and downstream validation plans.
✔️Strict Quality Control
QC at each step, including protein preparation, instrument performance, and data analysis, to ensure reliability and consistency.
✔️Collaborative Scientific Support
A professional proteomics team that can help interpret results, suggest follow-up studies, and support publication or regulatory documentation where appropriate.
Applications of Discovery Proteomics Service
Discovery Proteomics Service at MtoZ Biolabs supports a wide range of research areas, including but not limited to:
1. Disease mechanism research: cancer, inflammatory diseases, metabolic disorders, neurological diseases, cardiovascular conditions
2. Biomarker discovery: diagnostic, prognostic, and predictive markers in tissues and biofluids
3. Drug target identification and MOA studies: on-target and off-target effects, resistance mechanisms, toxicity signatures
4. Basic biology: signaling pathways, developmental processes, cell differentiation, stress responses
5. Microbiology and metaproteomics: host-microbe interactions, community function, microbial adaptation
6. Agricultural and plant research: stress tolerance, yield traits, plant-pathogen interactions, quality traits
FAQ
Q1: What types of samples are suitable?
We accept a broad range of samples for Discovery Proteomics Service, including but not limited to:
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Cultured cell pellets (adherent or suspension)
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Fresh or frozen animal and human tissues
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FFPE tissue curls or sections
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Biofluids such as plasma, serum, CSF, urine, saliva and others
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Microbial and plant samples
If you have special or limited material, you can contact us to confirm feasibility.
Q2: How should I prepare my samples?
1. Cells: Harvest, wash with PBS if possible, remove supernatant completely, and snap-freeze pellets.
2. Tissues: Rinse to remove excess blood when applicable, blot dry, snap-freeze small pieces, and store at −80°C.
3. Biofluids: Centrifuge to remove debris, aliquot supernatant, and freeze at −80°C.
4. FFPE: Thickness of 5-10 μm, size of 50 mm², with a minimum of 10 slices.
Transport samples on dry ice or ice packs to maintain stability during delivery and avoid freeze–thaw cycles.
Detailed requirements and recommendations can be found in the Sample Submission Guidelines for Proteomics.
Q3: What is the service general workflow?
Q4: What data formats are provided?
1. Raw data files in instrument-specific formats
2. Peptide and protein identification tables (qualitative results) in Excel or CSV
3. Quantitative result tables with normalized intensities, fold changes, and statistics in Excel or CSV
4. Key visualizations such as heatmaps, volcano plots, PCA and clustering plots in high-resolution image formats
5. A structured PDF report summarizing workflow, QC metrics, main findings, and biological interpretation
6. Additional or customized data formats can be provided upon request.
Start Your Project with MtoZ Biolabs
Discovery Proteomics Service at MtoZ Biolabs provides an end-to-end solution for unbiased, in-depth characterization of the proteome in your system of interest.
If you are planning a discovery proteomics project or considering how proteomics can support your current research, MtoZ Biolabs is ready to collaborate and provide high-quality, data-driven insights for your next breakthrough. Free project evaluation, welcome to learn more details!
