MtoZ Biolabs uses advanced mass spectrometry platforms to launch the 4D-PRM targeted proteomics analysis service which enables highly selective and highly sensitive quantification of low-abundance target proteins. This service is widely applied in targeted protein verification, signaling pathway studies, and precise quantification of functional proteins, providing a reliable data foundation for accurately understanding expression changes of specific proteins.
What Is 4D-PRM?
4D-PRM is a targeted quantitative proteomics method that combines parallel reaction monitoring with ion mobility technology, enhancing detection selectivity and sensitivity through four-dimensional information including m/z, retention time, ion mobility, and signal intensity. Its core principle is to precisely fragment target precursor ions after TIMS separation and monitor the product ions, enabling reliable quantification in complex backgrounds. 4D-PRM is widely used in targeted protein quantification, pathway validation, and marker studies, offering essential support for accurate protein analysis.
Kononikhin, A. et al. Analytical Chemistry, 2022.
Figure 1. Illustration of the prm-PASEF method.
4D-PRM Targeted Proteomics Analysis Service at MtoZ Biolabs
MtoZ Biolabs provides high-sensitivity, high-selectivity targeted protein quantification services using 4D-PRM targeted proteomics technology.
- Precise capture and monitoring of proteins
- Quantitative analysis of low-abundance target proteins in complex proteomes
- Evaluation of abundance changes in specific proteins
- Comparative analysis of target protein abundance across multiple sample groups
Our analysis offers a clear understanding of quantitative characteristics of target proteins, enabling researchers to obtain more reliable data support and advance their studies with confidence.
Workflow of 4D-PRM Targeted Proteomics Analysis Service
1. Sample Preprocessing
Perform protein extraction, quantification, and enzymatic digestion to obtain high-quality peptides suitable for targeted analysis.
2. Signature Peptide Selection
Select signature peptides based on target protein information and optimize the monitoring list using ion mobility parameters.
3. 4D-PRM Mass Spectrometry Acquisition
Conduct four-dimensional precise detection and fragmentation of target peptides using ion mobility separation combined with PRM mode.
4. Data Processing and Quantification
Use dedicated software to analyze four-dimensional data, extracting peak areas, retention times, and ion mobility values to achieve accurate quantification of target proteins.
5. Result Compilation and Reporting
Provide standardized result files and spectral plots, along with a summary and interpretation of quantitative outcomes for the target proteins.
Why Choose MtoZ Biolabs?
✅ Four-Dimensional Separation Capability: Combines m/z, retention time, ion mobility, and signal intensity to achieve higher selectivity.
✅ Low-Abundance Target Detection: Enables stable identification and quantification of low-abundance proteins even in complex matrices.
✅ High Quantification Accuracy: Precisely monitors signature peptides to ensure consistent and reliable quantification of target proteins.
✅ Reduced Background Interference: Ion mobility separation effectively minimizes co-eluting interference and enhances detection specificity.
✅ Customizable Analysis Strategy: Allows flexible adjustment of monitoring strategies and parameters based on target protein characteristics and research needs.
Applications of 4D-PRM Targeted Proteomics Analysis Service
1. Cell State Change Studies
Used to evaluate trends in key protein changes during specific treatments, stimuli, or environmental shifts.
2. Functional Correlation Verification
Used to determine whether observed biological changes align with variations in target protein levels, supporting further research.
3. Biological Process Monitoring
Suitable for tracking dynamic changes in key proteins during culture, differentiation, development, or stress responses.
4. Quality Consistency Assessment
Applied to compare sample consistency across different batches, workflows, or material sources.
5. Time-Series Monitoring Studies
Used to continuously monitor protein level changes at specific time points, supporting dynamic trend analysis.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Protein Quantitative Analysis Results
4. Related Mass Spectra and Visualized Charts
5. Bioinformatics Analysis
6. Raw Data Files
FAQ
Q1: What types of samples are suitable?
A1: The 4D-PRM targeted proteomics analysis service is suitable for proteomic samples with clear origin, good solubility, and basic purity, including cell lysates, tissue extracts, serum, plasma, and recombinant proteins. For low-abundance or complex-matrix samples, preprocessing can further improve targeted detection performance.
Q2: What is the service general workflow?
A2:
Q3: What data formats are provided?
A3: MtoZ Biolabs provides multiple standardized output formats, including:
- Mass spectrometry raw data files (such as .raw platform formats)
- Quantitative results of target proteins (Excel/CSV)
- PDF analysis report (including method description, data summary, and result interpretation)
- Visualization files (such as chromatographic peaks and fragment ion spectra, PNG/TIFF)
If special analytical requirements exist, data formats can be customized according to project specifications.
Q4: How should I prepare my samples?
A4: To ensure optimal analytical performance, please prepare samples as follows:
(1) Sample Purity: Avoid high salt, detergents, glycerol, and other components that may affect ionization or enzymatic digestion.
(2) Sample Storage: Store at 4℃ for short-term use; for long-term storage, keep at -80℃ and avoid repeated freeze-thaw cycles.
(3) Sample Shipment: Transport on dry ice or ice packs, and ensure sealed packaging to prevent leakage or contamination.
(4) Additional Information: Please provide sample type, concentration, buffer composition, and pretreatment steps so that we can develop the most appropriate analytical strategy.
For more information, please refer to Sample Submission Guidelines for Proteomics, Sample Submission Guidelines for Metabolomics.
Start Your Project with MtoZ Biolabs
Contact us to discuss your experimental design or request a quote. Whether you are exploring expression changes of specific proteins or studying differences between sample groups, we offer reliable and professional analytical support.
