MtoZ Biolabs uses a high-resolution Orbitrap mass spectrometry platform and offers the PRM targeted proteomics analysis service which enables highly sensitive and highly selective quantitative analysis of specific proteins. This service is suitable for multiple types of proteomics studies and provides reliable data support for the precise evaluation of target protein abundance changes.
What Is PRM?
PRM (parallel reaction monitoring) is a type of targeted quantitative technique based on high-resolution tandem mass spectrometry. Its principle is to select the precursor ion of the target peptide and then simultaneously collect all of its fragment ions, enabling precise quantification with high resolution and high specificity. Compared with SRM/MRM, which monitors only specific ion pairs, PRM improves result reliability and anti-interference capability through full-spectrum fragment acquisition. This method is commonly used for key protein quantification, low-abundance peptide detection, and validation of post-translational modification sites.
PRM Targeted Proteomics Analysis Service at MtoZ Biolabs
MtoZ Biolabs uses high-resolution PRM targeted mass spectrometry technology to provide clients with highly specific and highly accurate protein quantification services, supporting precise quantitative analysis of target proteins.
- High-resolution and precise quantification of target proteins
- Targeted detection of low-abundance proteins
- Quantification of common post-translationally modified proteins
- Quantitative comparison of target molecules across multiple sample groups
Our analysis provides a more comprehensive understanding of abundance changes and regulatory characteristics of target proteins, enabling researchers to obtain more robust validation evidence and further advance proteomics studies.
Workflow of PRM Targeted Proteomics Analysis Service
1. Sample Preparation
Perform protein extraction, enzymatic digestion, and necessary purification steps to ensure peptide quality meets PRM analysis requirements.
2. Target Selection and Method Establishment
Select signature peptides based on the target protein, determine optimal fragment ions, and establish a high-resolution PRM monitoring method.
3. Mass Spectrometry Acquisition
Use high-resolution tandem mass spectrometry to precisely capture selected ions and obtain high-fidelity fragment ion signals.
4. Data Analysis
Compare and quantify fragment ion peak shapes, retention times, and intensities to generate highly selective quantitative results.
5. Result Output
Provide standardized quantitative data, spectral plots, and a complete experimental report for subsequent validation or in-depth studies.
Rauniyar, N. International Journal of Molecular Sciences, 2015.
Figure 1. PRM Workflow.
Why Choose MtoZ Biolabs?
✅ Resolution Assessment: Relying on high-resolution platforms such as Orbitrap to provide higher selectivity and clearer fragment ion signals.
✅ High Specificity: By monitoring the complete set of fragment ions, improving the reliability of target identification and quantification.
✅ High Sensitivity: Accurately detecting low-abundance target peptides in complex backgrounds.
✅ Multi-Target Parallel Analysis: Able to simultaneously monitor multiple target peptides, suitable for large-scale targeted studies.
✅ Customized Analysis: Flexibly designing targets, fragment ions, and acquisition parameters according to research objectives.
Applications of PRM Targeted Proteomics Analysis Service
1. Biomarker Validation
Used for targeted quantification of candidate biomarkers, confirming expression differences and stability across different sample types.
2. Protein Function Studies
By precisely monitoring abundance changes of key proteins or their signature peptides, supporting the investigation of critical regulatory events in biological processes.
3. Signal Pathway Analysis
Applied to track core proteins and their dynamic expression levels within signaling pathways, enabling verification of essential pathway nodes.
4. Inter-Sample Comparison
Suitable for quantitative comparison of target proteins across multiple sample groups, helping to identify condition-specific or treatment-induced changes.
5. Quality Consistency Assessment
Used to evaluate the expression consistency of target proteins during sample preparation or experimental workflows, improving the reliability of research results.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Protein Quantification Results
4. Mass Spectra and Visualization Figures
5. Bioinformatics Analysis
6. Raw Data Files
FAQ
Q1: What types of samples are suitable?
A1: Suitable samples include soluble, clear, and relatively high-purity protein samples, such as cell lysates, tissue extracts, biofluids, and enriched or pretreated target proteins. Samples should minimize interference from salts, surfactants, and complex matrices to ensure the stability and accuracy of PRM-based targeted quantification.
Q2: What is the service general workflow?
A2:
Q3: What data formats are provided?
A3: MtoZ Biolabs provides multiple standardized outputs, including:
- Quantitative results of target proteins (XLSX/CSV)
- Mass spectra and visualization charts (PNG/TIFF)
- Method parameter files (including chromatographic conditions and PRM monitoring information)
- Comprehensive analysis report (PDF)
- Raw mass spectrometry data files (instrument-native format)
If special analytical requirements exist, data formats can be customized according to project specifications.
Q4: How should I prepare my samples?
A4: To ensure optimal identification performance, it is recommended to prepare samples as follows:
(1) Purity: Samples should be free of precipitation and turbidity, with controlled levels of salts, lipids, and surfactants
(2) Storage: Store at 4℃ for short-term use, and at -80℃ for long-term preservation; avoid repeated freeze-thaw cycles
(3) Transportation: Use sealed, leak-proof containers and ship under cold-chain conditions (ice packs/dry ice)
(4) Additional Information: Providing sample origin and target protein information is recommended to support optimization of monitoring methods and quantification workflows
For more information, please refer to Sample Submission Guidelines for Proteomics, Sample Submission Guidelines for Metabolomics.
Start Your Project with MtoZ Biolabs
Contact us to discuss your experimental design or request a quote. Whether you are focused on precise abundance changes of target proteins or validating quantitative differences of key molecules, we can provide stable and reliable targeted proteomics support.
