MtoZ Biolabs provides Targeted Proteome Quantification Service to support biomarker verification, translational research, and mechanistic studies across preclinical and clinical projects. Using targeted mass spectrometry and high-performance immunoassay platforms, we deliver precise, reproducible quantification of predefined protein panels in a wide range of biological matrices.
Overview
Targeted proteome quantification focuses on accurate, sensitive measurement of a selected set of proteins or peptides rather than profiling the entire proteome. Instead of discovering new proteins on a broad scale, targeted approaches concentrate analytical power on specific markers, pathways, or panels that are already of interest, such as disease biomarkers, drug targets, signaling nodes, or pathway readouts.
In contrast to discovery proteomics, which is primarily designed to maximize proteome coverage, targeted proteomics focuses on quantitative precision, wide dynamic range, and high reproducibility. By predefining transitions or precursor ions of interest, targeted workflows achieve:
- Higher sensitivity for low-abundance proteins
- Wider linear dynamic range suitable for clinical and translational studies
- Lower batch-to-batch variability, ideal for longitudinal and large cohort studies
- Direct quantitative readouts that can be linked to pharmacodynamic effects, disease activity, or treatment response
Targeted Proteome Quantification Service at MtoZ Biolabs
💠SRM/MRM Targeted Proteomics Analysis Service
Triple quadrupole-based SRM/MRM assays for precise, high dynamic range quantification of predefined peptide panels in complex biological samples.
Guerrero, L. N. et al. Metabolites. 2022.
Figure 1. Multiple/Selected Reaction Monitoring (MRM/SRM) Targeted Proteomics
💠PRM Targeted Proteomics Analysis Service
High-resolution PRM workflows acquire full fragment ion spectra for each targeted precursor, providing greater selectivity, specificity, and post acquisition refinement for quantifying peptides and PTM sites in complex biological matrices.
Rauniyar, N. Int J Mol Sci. 2015.
Figure 2. PRM Workflow
💠4D-PRM Targeted Proteomics Analysis Service
Ion mobility enhanced PRM that adds a mobility dimension to improve separation, reduce interferences, and boost sensitivity for low-abundance targets.
Kononikhin, A. et al. Analytical Chemistry. 2022.
Figure 3. Illustration of the PRM-PASEF Method
💠DIA+PRM Targeted Proteomics Services
Integrated DIA discovery and PRM validation to screen broad panels first and then refine and quantify key proteins with higher precision.
Figure 4. The Workflow of DIA+PRM Targeted Proteomics Analysis
💠Olink Proteomics Profiling Service
Proximity extension-based panels that enable high-throughput, low volume quantification of hundreds of proteins in plasma, serum, CSF, and other biofluids.
Carlyle, B. C. et al. Frontiers in Neurology. 2022.
Figure 5. Olink PEA Technology Uses Oligonucleotide-Conjugated Antibody Pairs for Targeting Different Epitopes of the Same Protein
💠Electrochemiluminescence (ECL) Assay Service
Plate based ECL immunoassays for multiplexed, highly sensitive measurement of cytokines and other soluble protein biomarkers in research and translational studies.
Zanut, A. et al. Nature Communications, 2020.
Figure 6. Schematic Representation of the Electrochemiluminescence (ECL) Immunoassay (Sandwich Assay)
Why Choose MtoZ Biolabs
☑️High Analytical Specificity and Sensitivity: Carefully optimized transitions, acquisition parameters, and response curves for confident quantification of low-abundance proteins and PTMs.
☑️Assay Design and Optimization Support: Assistance with panel design, peptide and antibody selection, cutoff strategy, and pilot feasibility studies based on your biological questions.
☑️Rigorous QC and Reproducibility: Standardized workflows, internal controls, and repeat measurements to ensure robust performance across batches, and study phases.
☑️Collaborative Expert Team: Proteomics and bioinformatics specialists work with you from study design through data interpretation to deliver decision-ready results.
Applications of Targeted Proteome Quantification Service
Targeted proteome quantification has become an essential tool in:
- Biomarker verification and panel refinement after discovery screens
- Pharmacokinetic and pharmacodynamic (PK PD) studies
- Companion biomarker development and patient stratification
- Mechanism of action (MOA) elucidation and pathway monitoring
- Quality control and lot release testing of biological products
FAQ
Q1: What types of samples are suitable?
We support a wide range of matrices for targeted proteome quantification, including but not limited to:
- Human and animal serum or plasma
- Other biofluids such as urine, CSF, saliva, synovial fluid, and cell culture supernatant
- Fresh or frozen tissues and tissue lysates
- Isolated cells or cell pellets from in vitro or in vivo studies
Available targets and panel performance may vary by matrix, so we will work with you to confirm feasibility for your specific sample type.
Q2: How should I prepare my samples?
1. Ensure samples are freshly prepared or snap-frozen.
2. Avoid detergents, salts, and reducing agents that may interfere with LC-MS/MS analysis.
3. Store samples at –80 °C before shipment.
4. Transport samples on dry ice to maintain stability during delivery.
Detailed requirements and recommendations can be found in the Sample Submission Guidelines for Proteomics.
Q3: What is the service general workflow?
Q4: What data formats are provided?
MtoZ Biolabs offers standardized data formats, including:
- Target protein quantification results table (XLSX/CSV)
- Mass spectrometry spectra and visualization charts (PNG/TIFF)
- Method parameter files
- Comprehensive analysis report (PDF)
- Raw mass spectrometry data files (instrument-compatible formats)
If special analytical requirements exist, data formats can be customized according to project specifications.
Start Your Project with MtoZ Biolabs
With MtoZ Biolabs as your partner for Targeted Proteome Quantification Service, you gain access to integrated technologies and expert support. Our technical specialists are available to provide a free business assessment.
