MtoZ Biolabs utilizes advanced instruments and analytical platforms to offer N-terminal and C-terminal sequencing services which providing precise analysis of the N-terminal and C-terminal amino acid sequences of proteins. This service is widely used in proteomics, antibody engineering, recombinant protein research, and biomarker discovery, providing reliable data support for protein functional analysis, quality control, and structural studies.

Overview

The N-terminal and C-terminal of proteins are the amino and carboxyl ends, marking the protein's start and termination. The N-terminal and C-terminal are critical to protein function and stability, with the N-terminal sequence determining protein folding and degradation, and the C-terminal involved in protein interactions. Sequencing the N-terminal and C-terminal sequences helps confirm protein integrity, identify mutations or truncations, and is widely used in antibody engineering, protein modification analysis, and drug development, helping to deepen the understanding of protein structure and function.

N-Terminal and C-Terminal Sequencing Services at MtoZ Biolabs

1. Edman Degradation Method

The Edman degradation method determines the amino acid sequence by sequentially cleaving the N-terminal amino acids and reading the derivative signals. This method directly reads the sequence from the protein's N-terminus and does not rely on a database. It is suitable for high-purity protein samples and is widely used for N-terminal sequencing.

2. Mass Spectrometry Method

Mass spectrometry, combined with liquid chromatography (LC-MS), analyzes the amino acid sequence of the N-terminus or C-terminus by obtaining fragment ion signals from the protein. This method has high sensitivity for complex samples and efficiently reads N-terminal and C-terminal sequence information, making it suitable for the precise analysis of various proteins and peptide sequences.

Figure 1. N-Terminal nd C-Terminal Sequencing Workflow Based on Mass Spectrometry and Edman Degradation.

Why Choose MtoZ Biolabs?

• High Coverage: Multiple analytical techniques are employed to ensure full coverage of both N-terminal and C-terminal sequences.
• Fast and Accurate: Optimized workflows reduce sample analysis time while maintaining high accuracy of results.
• Strong Reproducibility: Standardized procedures ensure consistency of results across different batches and experiments.
• Customized Analysis: Sequencing strategies can be tailored based on the protein type and research objectives.
• One-stop Service: Provides a complete analysis process from sample evaluation to report delivery.

Applications of N-Terminal and C-Terminal Sequencing Services

1. Engineering Protein Confirmation

Confirming the N-terminal/C-terminal sequence consistency of recombinant or fusion proteins to ensure the accuracy of the engineered sequence.

2. Biomarker Discovery

Extracting the N-terminal/C-terminal sequences of potential biomarkers from clinical samples to aid in screening for potential biomarkers.

3. Quality Control

Checking the N-terminal/C-terminal sequence consistency of proteins in production batches to ensure batch-to-batch consistency.

4. Drug Development

Analyzing the N-terminal/C-terminal sequences of candidate drug molecules to support drug target validation and molecular characteristic confirmation.

5. Signal Pathway Analysis

Analyzing the N-terminal/C-terminal sequences of key proteins in signal pathways to help study the activity of signaling molecules and their roles in cells.

Deliverables

• Comprehensive Experimental Details
• Materials, Instruments, and Methods
• N-terminal/C-terminal Sequence Analysis Results Table
• Mass Spectrometry Images and Visualized Charts
• Raw Data Files
• Comprehensive Analysis Report

FAQ

Q1: What types of samples are suitable?

A1: Suitable samples include soluble, clear, and high-purity proteins, such as recombinant proteins, fusion proteins, purified proteins, and related protein fragments. Samples should avoid high salt, highly viscous matrices, and particulate interference to ensure the stability and accuracy of mass spectrometry analysis.

Q2: What is the service general workflow?

A2:

Q3: What data formats are provided?

A3: MtoZ Biolabs provides the following standard data formats:

• Raw data (XLSX/CSV/TXT)

• N-terminal/C-terminal sequence analysis result tables (XLSX/CSV/TXT)

• Mass spectrometry images and visualized figures (PNG/TIFF)

• Comprehensive analysis report (PDF), including experimental methods, parameters, and sequence conclusions

If special analytical requirements exist, data formats can be customized according to project specifications.

Q4: How should I prepare my samples?

A4: It is recommended to prepare and submit samples following the guidelines below:

• Purity: Ensure that the solution has no precipitate or turbidity, and avoid impurity interference
• Storage: Store at 4℃ for short term and -80℃ for long term, and avoid repeated freeze-thaw cycles
• Transportation: Use cold-chain transport (ice packs/dry ice), ensure proper sealing, and avoid particulate contamination
• Additional Information: Please provide key information such as sample source, estimated concentration, and solvent composition to support analysis optimization.

For more information, please refer to Sample Submission Guidelines for Proteomics, Sample Submission Guidelines for Metabolomics.

Start Your Project with MtoZ Biolabs    

Contact us to discuss your experimental design or request a quote. Whether you are exploring N-terminal and C-terminal sequence features of proteins or studying protein function and structure, MtoZ Biolabs can provide you with precise sequence analysis support.