MtoZ Biolabs offers Fecal Proteomics Solutions leveraging high-resolution mass spectrometry to analyze proteins in fecal samples for clinical and translational research. Our service ensures comprehensive protein identification, accurate quantification, and functional insights, advancing biomarker discovery and gut-proteome studies for various gastrointestinal and metabolic disorders.
Introduction
The proteome of feces reflects a complex mixture of host-derived proteins, enzymes, immune mediators, and microbial proteins, providing valuable insights into gastrointestinal health and disease. This non-invasive method allows researchers to monitor the molecular signatures of the gut microbiota, gut barrier function, and the immune system's response to various environmental and pathological stimuli.
By analyzing protein changes in feces, it is possible to detect early biomarkers for disease onset, monitor disease progression, and evaluate therapeutic responses. The diversity of proteins found in fecal samples makes this an ideal tool for studying the dynamic interaction between the gut microbiome and the host, as well as identifying potential biomarkers for early disease diagnosis and personalized treatment strategies.
Lee, PY. et al. J Biomed Sci. 2017.
Figure 1. Applications of Human Fecal Proteomics
Fecal Proteomics Solutions at MtoZ Biolabs
MtoZ Biolabs offers comprehensive Fecal Proteomics Solutions, utilizing advanced DIA (Data-Independent Acquisition) mass spectrometry to provide detailed protein profiling from fecal samples. This approach enables precise identification and quantification of proteins, including low-abundance species, delivering accurate and unbiased analysis of complex gut samples. By applying DIA-MS technology, we ensure a deeper understanding of the dynamic changes in the gut microbiome and its relationship with overall health.
Our service can detect up to 9,000 proteins per sample, providing high-throughput data for both clinical and fundamental research applications. We offer a full spectrum of proteomic analyses, from global proteome mapping to targeted proteomics, where specific protein panels can be customized for in-depth quantification. Whether for biomarker discovery, disease monitoring, or microbiome-related studies, MtoZ Biolabs ensures comprehensive and reliable data to support your research goals.
Workflow of Fecal Proteomics Solutions
1. Sample Collection and Preparation
Fecal samples are collected and immediately snap-frozen to preserve protein integrity. They are then homogenized to ensure uniformity for proteomic analysis.
2. Protein Extraction
Proteins are extracted from the homogenized fecal samples using optimized buffers that minimize contamination and preserve low-abundance proteins.
3. Enzymatic Digestion
Proteins are digested using sequencing-grade enzymes, generating peptides suitable for mass spectrometry analysis.
4. Peptide Desalting and Cleanup
Desalting and cleanup steps remove salts and contaminants to ensure compatibility with UPLC-MS/MS.
5. LC-MS/MS Analysis
Peptides are separated by UPLC and analyzed using DIA-MS for high-resolution, unbiased protein identification and quantification.
6. Data Processing and Analysis
Mass spectrometry data is processed to identify proteins, quantify their abundance, and perform functional annotation. Statistical analysis, pathway enrichment, and protein interaction networks are also included.
Why Choose MtoZ Biolabs
✔️High-Precision Analysis
Sensitive detection of low-abundance proteins ensures accurate and reliable results.
✔️Comprehensive Coverage
Detects up to 9,000 proteins per sample, including trace and low-abundance proteins.
✔️High Throughput
Efficient processing of large-scale samples ensures reproducibility and data consistency.
✔️Customizable
Tailored proteomics analysis with custom protein panels for specific research needs.
✔️Experienced Team
Expert proteomics team provides reliable data interpretation and guidance.
✔️Stable and Reliable
Consistent results across analyses, providing high-confidence data for research.
Applications of Fecal Proteomics Solutions
1. Gut Disease Biomarkers
Identifying protein markers for conditions like inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), and colorectal cancer.
2. Gut Microbiome Research
Exploring protein interactions between the gut microbiota and host, including immune responses and microbial protein profiles.
3. Gastrointestinal Infections
Studying protein shifts in response to bacterial or viral infections in the gut, aiding in disease mechanisms and therapeutic targets.
4. Metabolic and Mucosal Health
Examining proteins related to gut barrier function, metabolic disorders, and mucosal immunity.
5. Therapeutic Monitoring
Tracking protein changes to assess treatment efficacy in gastrointestinal diseases or microbiome-targeted therapies.
FAQ
Q1: What types of samples are suitable?
We support multiple sample types, including:
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Fecal samples collected from humans or animal models
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Microbial pellets or isolates derived from feces
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Cultured cells related to gut or microbiome studies
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Purified proteins extracted from feces, microbes, or gut-related sources
Q2: How should I prepare my samples?
1. Collect samples into low-binding tubes or appropriate sterile containers.
2. Snap-freeze immediately after collection to preserve protein integrity.
3. Avoid detergents, high salts, and strong reducing agents that can interfere with LC-MS/MS.
4. Store at -80 °C and ship on dry ice to maintain stability during transport.
Detailed requirements and recommendations can be found in the Sample Submission Guidelines for Proteomics.
Q3: What is the service general workflow?
Q4: What data formats are provided?
1. Raw MS files (.raw, .d, .wiff, depending on instrument).
2. Quantitative result tables in .xlsx or .csv, including protein intensity and key statistics.
3. Bioinformatics outputs such as enrichment and network results in .xlsx or .csv.
4. Publication-ready figures in high-resolution .png or .tiff.
5. A complete PDF report summarizing methods, QC metrics, and main findings.
6. Additional formats are available upon request.
Start Your Project with MtoZ Biolabs
MtoZ Biolabs offers Fecal Proteomics Solutions optimized for challenging gut secretion matrices, ensuring high mass accuracy and throughput. Free project evaluation, welcome to learn more details.
