MtoZ Biolabs utilizes high-resolution mass spectrometry platform to offer the full-length protein sequence analysis service which provides precise analysis of the complete amino acid sequence of proteins. This service is widely applied in proteomics, recombinant protein research, antibody engineering, and biomarker discovery, providing solid data support for in-depth studies on protein function and structure.

Overview

The full-length protein sequence refers to the complete amino acid arrangement from the N-terminal to the C-terminal of the protein, reflecting its overall structure and functional potential. Full-length sequence analysis reveals the true amino acid sequence of the protein through precise sequencing, aiding in confirming protein integrity, functional regions, and post-translational modification sites. This service is widely used in antibody development, protein engineering, disease research, and drug screening, offering reliable sequence data support for scientists and facilitating a deeper understanding of the biological functions and mechanisms of proteins.

Mai, Z. et al. Anal Chem. 2022.

Figure 1. Protein Full-Length Sequencing.

Full-Length Protein Sequene Analysis Service at MtoZ Biolabs

MtoZ Biolabs offers full-length protein sequence analysis service through its high-resolution mass spectrometry platform, providing customers with complete amino acid sequence analysis of proteins.

• Confirming the full amino acid sequence of the protein

• Identifying mutations and variants in the protein sequence

• Analyzing the impact of post-translational modifications on the protein sequence

• Verifying sequence consistency between recombinant and native proteins

Our analysis provides a comprehensive understanding of the full-length protein sequence and its functional characteristics, enabling researchers to obtain reliable support in proteomics research, quality control, and biomarker discovery.

Workflow of Full-Length Protein Sequene Analysis Service

1. Sample Preparation

Dissolve, purify, and perform necessary desalting on the protein samples to ensure they are stable, clear, and suitable for subsequent mass spectrometry analysis.

2. Enzymatic Digestion and Peptide Generation

Use appropriate proteases to cleave the protein into peptides suitable for analysis, ensuring uniform peptide generation for mass spectrometry.

3. Mass Spectrometry Analysis

Utilize high-resolution mass spectrometry techniques (e.g., LC-MS/MS) to analyze peptides and acquire accurate fragment ion signals, providing the foundation for sequence analysis.

4. Data Processing and Analysis

Analyze the mass spectrometry data to deduce the full-length protein sequence and identify variants or mutations.

5. Report Generation

Provide a detailed analysis report, including the complete amino acid sequence, variants, and modifications, along with the methods and experimental parameters.

Why Choose MtoZ Biolabs?

• High Coverage: Capable of reading the full-length protein sequence comprehensively, ensuring the integrity of the sequence.
• High Sensitivity: Able to process low-abundance and trace samples, suitable for protein analysis in complex systems.
• High Reliability: Uses standardized procedures and multiple validations to ensure the high accuracy of the analysis results.
• Customized Analysis Plans: Tailored analysis strategies based on different protein types and research needs.
• Fast and Efficient Delivery: Provides efficient and rapid analysis services, shortening research timelines.

Applications of Full-Length Protein Sequene Analysis Service

1. Biomarker Discovery

By analyzing the full-length sequences of proteins in clinical samples or body fluids, it aids in the screening of potential biomarkers.

2. Antibody Engineering

Used to analyze the full-length sequences of antibodies and their derivative proteins, ensuring antibody consistency and quality.

3. Immune Response Analysis

Analyzing the full-length sequences of key proteins in immune responses, helping to understand immune system functions and antigen recognition mechanisms.

4. Cell Signaling Research

Used to analyze the full-length sequences of key proteins in signal transduction, helping to study how cells respond to internal and external signals.

5. Protein Modification and Functional Research

Full-length sequence analysis can identify the specific locations of protein post-translational modifications, providing deeper data support for functional studies.

Deliverables

• Comprehensive Experimental Details
• Materials, Instruments, and Methods
• Full-Length Protein Sequence Analysis Results
• Mass Spectra Images and Spectral Data
• Raw Data Files
• Comprehensive Analysis Report

FAQ

Q1: What types of samples are suitable?

A1: Suitable for soluble, clarified, and high-purity protein samples, including recombinant proteins, fusion proteins, purified proteins, and native proteins. Samples should avoid high salt, high viscosity, or particulate interference to ensure stability and accuracy in mass spectrometry analysis.

Q2: What is the service general workflow?

A2:

Q3: What data formats are provided?

A3: MtoZ Biolabs provides the following standard data formats:

• Full-length protein sequence result tables (XLSX/CSV format)

• Mass spectrometry images and spectra (PNG/TIFF format)

• Raw data lists (XLSX/CSV format)

• Comprehensive analysis reports (PDF format)

If special analytical requirements exist, data formats can be customized according to project specifications.

Q4: How should I prepare my samples?

A4: To ensure optimal identification, we recommend preparing samples as follows:

• Purity: Ensure the protein solution is free of precipitation and turbidity, and minimize impurity interference.
• Storage: Store short-term at 4℃ and long-term at -80℃ to avoid repeated freezing and thawing.
• Shipping: Use cold chain (ice packs or dry ice) with leak-proof packaging.
• Additional Information: Provide sample origin, concentration estimation, buffer composition, etc., to facilitate method optimization and adjustments in the analysis process.

For more information, please refer to Sample Submission Guidelines for Proteomics, Sample Submission Guidelines for Metabolomics.

Start Your Project with MtoZ Biolabs    

Contact us to discuss your experimental design or request a quote. Whether you are exploring the completeness of protein sequences or studying protein functions and characteristics, MtoZ Biolabs provides precise sequence analysis support.