MtoZ Biolabs utilizes high-resolution LC-MS/MS mass spectrometry platforms to offer the mass spectral protein sequencing service which providing comprehensive and accurate protein sequence analysis. This service is suitable for unknown sequence confirmation, engineered protein evaluation, and biomarker research, providing reliable data support for protein structure and function analysis.

Overview

Protein sequencing is the process of analyzing the actual amino acid arrangement of proteins, which can be used to confirm molecular identity and identify sequence variants. Mass spectrometry-based sequencing uses enzyme digestion to obtain peptides and analyzes the mass differences between fragment ions to deduce the residue sequence, enabling rapid and reliable sequence information acquisition. This method is highly sensitive and versatile, commonly used in recombinant protein validation, antibody engineering, biomarker discovery, and mechanism research, making it an efficient and important analytical tool in proteomics.

Mass Spectral Protein Sequencing Service at MtoZ Biolabs

MtoZ Biolabs utilizes high-resolution mass spectrometry sequencing technology to provide precise analysis of protein sequences and related structural features.

• Obtaining the true amino acid sequence of proteins

• Identifying sequence variants, mutation sites, and potential truncations

• Analyzing the impact of common post-translational modifications on sequence fragments

• Verifying sequence consistency in recombinant proteins, antibodies, or engineered proteins

• Analyzing sequence composition and characteristics of target proteins in complex systems

Our analysis provides a systematic understanding of protein sequence composition and key structural information, enabling researchers to more efficiently advance structural research, functional mechanism exploration, and related application development.

Workflow of Mass Spectral Protein Sequencing Service

1. Sample Preparation

Dissolve, desalinate, or pre-purify the protein sample to ensure the solution is clear and suitable for the mass spectrometry workflow.

2. Peptide Digestion

Use specific proteases to digest the protein into readable peptide fragments, providing foundational information for sequence analysis.

3. Mass Spectrometry Acquisition

Capture fragmentation ion signals of the peptides using a high-resolution platform, generating stable spectra for sequence derivation.

4. Data Analysis

Reconstruct the amino acid sequence based on the mass differences between fragment ions, identifying sequence variants, modifications, and other critical information.

5. Report Generation

Integrate sequencing results, spectral evidence, and method parameters, providing a standardized protein sequence determination report.

Coon, J J. et al. BioTechniques, 2018.

Figure 1. Mass Spectrometry (MS)-Based Protein Sequencing.

Why Choose MtoZ Biolabs?

• High-Precision Sequence Analysis: Accurate amino acid sequencing is achieved through the precise mass difference between high-resolution fragmentation ions.
• Deep Coverage Performance: Multi-enzyme digestion and repeated fragmentation strategies enhance coverage of difficult-to-sequence regions.
• Low-Abundance Sample Compatibility: Maintains high sensitivity for low-abundance proteins and small volume samples.
• Flexible Customization: Experimental plans can be customized based on sample type and research objectives.
• One-Stop Delivery: Covers sample evaluation, mass spectrometry acquisition, sequence analysis, and comprehensive report generation.

Applications of Mass Spectral Protein Sequencing Service

1. Mutation and Variant Identification

By analyzing protein sequences, mismatches, mutations, truncations, and structural changes can be detected, supporting functional studies and quality assessments.

2. Biomarker Research

Used for sequence identification and feature extraction of candidate biomarker proteins in clinical samples or body fluids.

3. Modification Residue Determination

Identifying key post-translational modification sites aids in the study of activity regulation and structural stability.

4. Quality Control and Batch-to-Batch Consistency

Used to confirm the stability and consistency of protein sequences across production, expression, or purification batches.

5. Signal Pathway Research

Deciphering the sequence characteristics and functional regions of key protein fragments in signaling cascades contributes to the study of signaling pathways.

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Protein Sequence Analysis Results

4. MS/MS Fragmentation Spectra and Visual Images

5. Raw Data Files

6. Comprehensive Analysis Report

FAQ

Q1: What types of samples are suitable?

A1: Suitable samples include soluble, clarified, and high-purity proteins, such as recombinant proteins, antibodies, fusion proteins, purified protein complexes, peptides, and native proteins. Samples should avoid high salt, sticky matrices, and particle interference to ensure the stability and accuracy of mass spectrometry analysis.

Q2: What is the service general workflow?

A2:

Q3: What data formats are provided?

A3: MtoZ Biolabs provides various standardized data formats, including:

• Raw mass spectrometry data files (instrument-compatible formats)

• Protein sequence result tables (XLSX/CSV)

• Mass spectrometry images and fragmentation spectra (PNG/TIFF)

• Comprehensive analysis reports (PDF)

If special analytical requirements exist, data formats can be customized according to project specifications.

Q4: How should I prepare my samples?

A4: To ensure optimal identification results, samples should be prepared as follows:

• Purity: The protein solution should be free of precipitates and cloudiness to avoid interference from impurities.
• Storage: Store samples at 4℃ for short-term, -80℃ for long-term, and avoid frequent freeze-thaw cycles.
• Transportation: Ship samples under cold chain conditions, ensuring they are sealed to prevent leakage and reduce particle contamination.
• Additional Information: Please provide sample source, concentration estimates, and solvent composition to assist in optimizing the analysis process.

For more information, please refer to Sample Submission Guidelines for Proteomics, Sample Submission Guidelines for Metabolomics.

Start Your Project with MtoZ Biolabs    

Contact us to discuss your experimental design or request a quote. Whether you're exploring the unknown patterns of protein sequences or studying the details of protein function and structure, MtoZ Biolabs can provide you with precise mass spectrometry-based protein sequencing analysis support.