MtoZ Biolabs provides S-Nitrosoproteomics Service by combining high-resolution mass spectrometry analysis with selective enrichment strategies to comprehensively characterize protein S-nitrosylation profiles. This service helps research teams investigate nitric oxide (NO)-mediated signaling, redox regulation, and disease-related mechanisms, delivering reliable residue-level modification evidence and quantitative results.

What is Protein S-Nitrosylation

Protein S-nitrosylation is a reversible post-translational modification mediated by nitric oxide (NO), in which an S-NO bond forms on cysteine residues to regulate protein activity, conformation, and interactions.

Under physiological conditions, S-nitrosylation participates in signal transduction, metabolic regulation, and stress responses.

Under pathological conditions, its dysregulation is closely associated with cancer, cardiovascular disorders, and neurodegenerative diseases.

Due to its dynamic and low-abundance nature, along with environmental sensitivity, comprehensive analysis requires high-sensitivity mass spectrometry approaches.

Ye, H. et al. J Med Chem. 2022.

Figure 1. Workflows for Proteome-Wide SNO Detection

S-Nitrosoproteomics Service at MtoZ Biolabs

The S-nitrosoproteomics service offered by MtoZ Biolabs provides an end-to-end solution that includes sample preparation, enrichment, high-resolution mass spectrometry analysis, and bioinformatics interpretation. Our service includes:

• S-NO site identification: Detection and localization of cysteine S-nitrosylation sites.
• Quantitative analysis: Label-based (e.g., TMT) or label-free quantification of S-NO modifications under different conditions.
• Pathway and functional enrichment: Analysis of nitrosylated proteins involved in signal regulation and stress response.
• Customized project design: Selection of enrichment strategies and mass spectrometry platforms tailored to research objectives.

Why Choose MtoZ Biolabs

1.Advanced Analytical Platforms

Equipped with Thermo Fisher Q Exactive HF and Orbitrap Fusion Lumos systems.

2.Multi-Strategy Enrichment System

Supports Biotin Switch, Organomercury Resin, and Phosphine Probe approaches.

3.Strict Quality Control

Residue-level FDR control and multi-dimensional data validation.

4.Flexible Customization

Adjustable detection depth and quantification strategy based on sample type and research goals.

5.Extensive Expertise

Years of experience in proteomics and PTM analysis, ensuring accurate detection of low-abundance SNO signals.

Applications of S-Nitrosoproteomics Service

1. Cancer Research

Elucidating tumor signaling pathways and metabolic reprogramming regulated by S-NO modification.

2. Cardiovascular Diseases

Investigating the roles of S-nitrosylation in myocardial ischemia and vascular relaxation.

3. Neurodegenerative Disorders

Studying SNO-mediated dysfunction in Alzheimer’s and Parkinson’s diseases.

4. Immunity and Inflammation

Exploring NO-mediated immune regulation and inflammatory signaling networks.

5. Drug Mechanism Studies

Assessing molecular targets and effects of NO donors and antioxidant compounds.

Start Your Project with MtoZ Biolabs

Contact us today to start your protein S-nitrosylation research project with MtoZ Biolabs.

FAQs

Q1: What is the service's general workflow?

We accept cell lysates, tissue supernatants, membrane proteins, immunoprecipitated complexes, and purified proteins. For complex samples, pre-enrichment is recommended to improve detection sensitivity.

Q2: What is the service's general workflow?

Q3: What data formats are provided?

MtoZ Biolabs provides results in multiple standardized formats compatible with downstream bioinformatics and visualization platforms. Deliverables typically include:

• Raw data files from LC-MS/MS platforms (.raw or .mzML);

• Processed data tables (.csv or .xlsx) containing identified S-nitrosylated sites, peptide sequences, and quantitative values;

• Comprehensive reports in PDF format, including analytical methods, data quality metrics, and biological interpretation;

• Figures and spectra in high-resolution image formats (.TIFF or .PNG).

Additional file formats can be provided upon request to meet specific publication or database submission requirements.

Q4: How should I prepare my samples?

To ensure precise and reproducible S-nitrosoproteomics results, MtoZ Biolabs recommends the following preparation guidelines:

• Sample Type: Suitable materials include cell lysates, tissue homogenates, plasma, serum, or purified proteins.
• Sample Purity: Samples should be free from particulates, high salt, detergents (especially SDS), or organic solvents that may interfere with reduction or ionization.
• Protein Concentration: A minimum of 100 μg of total protein per sample is recommended; additional material may be required for replicate or quantitative analyses.
• Storage and Shipping: Store samples at −80°C and ship on dry ice in sealed, leak-proof containers. Avoid repeated freeze–thaw cycles.
• Documentation: Include detailed metadata such as sample origin, treatment conditions, extraction buffer composition, and experimental grouping.

For more information, please refer to Sample Submission Guidelines for Proteomics and Sample Submission Guidelines for Metabolomics.