MtoZ Biolabs provides Nitrosation Omics Service by integrating high-resolution mass spectrometry with advanced enrichment and labeling chemistries to systematically characterize nitrosation-based post-translational modifications (PTMs) across the proteome. This service enables research teams to explore nitric oxide (NO)-related redox signaling, protein regulation, and pathological processes, providing residue-level modification mapping, quantitative data, and functional annotation.
What is Protein Nitrosation
Protein nitrosation refers to the covalent attachment of a nitric oxide (NO) or nitroso group to nucleophilic amino acid residues, such as cysteine (S-nitrosation) or amine-bearing residues (N-nitrosation).
S-nitrosation (Protein S-nitrosylation) forms an S-NO bond on cysteine thiols, representing the most biologically relevant and reversible type of nitrosation.
N-nitrosation involves the modification of amine or amide groups, often contributing to stress response and, in some cases, nitrosamine formation.
These reversible modifications regulate protein structure, enzyme activity, and protein–protein interactions. Dysregulation of nitrosation is associated with oxidative stress, cancer, cardiovascular dysfunction, and neurodegenerative disorders.
Nitrosation Omics Service at MtoZ Biolabs
MtoZ Biolabs’ nitrosation omics service offers end-to-end workflows covering sample preparation, nitrosated peptide enrichment, LC-MS/MS analysis, and bioinformatics interpretation. Our service includes:
- Comprehensive nitrosation site mapping: Identification of both S- and N-nitrosated residues across the proteome.
- Quantitative profiling: Label-free or TMT-based quantification to compare modification levels under different physiological or pathological conditions.
- Functional interpretation: Pathway enrichment and redox network analysis to uncover NO-related biological regulation.
- Custom experimental design: Selection of labeling and enrichment strategies tailored to the research objective.
Workflow of Nitrosation Omics Service
1. Sample Preparation
Extraction of total proteins and stabilization of nitrosated residues to prevent oxidation or reduction artifacts.
2. Selective Reduction and Labeling
Use of ascorbate-dependent or phosphine-based reagents to selectively reduce nitrosated bonds, followed by labeling with biotin or affinity tags.
3. Affinity Enrichment
Capture of labeled peptides using streptavidin, mercury resin, or thiol-reactive probes.
4. LC-MS/MS Analysis
High-resolution tandem mass spectrometry using Orbitrap or timsTOF platforms to identify nitrosation sites.
5. Bioinformatics and Quantification
Data processing, site localization, differential quantification, and pathway enrichment analysis (GO/KEGG).
Why Choose MtoZ Biolabs
1.Comprehensive Omics Coverage
Analysis of both S- and N-nitrosation events across proteomic samples.
2. Advanced Instrumentation
Thermo Fisher Q Exactive HF and Orbitrap Fusion Lumos ensure high mass accuracy and sensitivity.
3.Multi-Strategy Enrichment System
Supports Biotin Switch, Organomercury Resin, and Phosphine Probe chemistries.
4.Rigorous Data Validation
Residue-level FDR control, replicates, and orthogonal verification.
5.Experienced Team
Decades of proteomics and PTM expertise ensure robust data interpretation and reliable biological conclusions.
Applications of Nitrosation Omics Service
1. Cancer Biology
Characterization of nitrosation-mediated tumor signaling and redox imbalance.
2. Cardiovascular Research
Investigating endothelial NO signaling, vasodilation control, and ischemia-related mechanisms.
3. Neurodegenerative Diseases
Mapping nitrosation-driven dysfunction of neuronal proteins in Alzheimer’s or Parkinson’s disease.
4. Inflammation and Immunity
Profiling NO-modified proteins involved in immune response and oxidative stress regulation.
5. Drug Mechanism Studies
Evaluating NO donors, antioxidants, or redox-modifying drugs at the proteome level.
Start Your Project with MtoZ Biolabs
Contact MtoZ Biolabs today to initiate your protein nitrosation omics project and obtain comprehensive insights into nitric oxide–based protein regulation.
FAQs
Q1: What types of samples are suitable?
Acceptable samples include cell lysates, tissue homogenates, plasma, serum, and purified proteins. For complex biological matrices, pre-enrichment or depletion may be recommended to improve detection sensitivity.
Q1: What is the service's general workflow?
Q3: What data formats are provided?
MtoZ Biolabs provides multiple standardized formats compatible with bioinformatics and visualization tools:
- Raw mass spectrometry files (.raw or .mzML);
- Processed data tables (.csv or .xlsx) containing identified nitrosation sites and quantitative data;
- Comprehensive analytical reports in PDF format;
- High-resolution images of chromatograms and spectra (.TIFF or .PNG).
Additional formats can be customized upon request.
Q4: How should I prepare my samples?
To ensure accurate and reproducible results:
- Sample Type: Submit cell lysates, tissue homogenates, plasma, or purified proteins.
- Purity Requirements: Avoid particulates, detergents (especially SDS), or high-salt buffers.
- Protein Quantity: Provide at least 100 μg total protein per sample; more for replicates.
- Storage and Shipping: Store at -80°C and ship on dry ice in leak-proof containers; avoid repeated freeze–thaw cycles.
- Documentation: Include sample origin, preparation method, buffer composition, and experimental design.
For more information, please refer to Sample Submission Guidelines for Proteomics and Sample Submission Guidelines for Metabolomics.