MtoZ Biolabs provides cutting-edge Protein Lipidation Analysis Service to help researchers study the critical role of lipidation in cellular processes. Lipidation is a key post-translational modification that affects protein function, localization, and interactions, and it plays a vital role in various biological pathways including cell signaling, metabolism, and immune responses. Our services deliver high-resolution, accurate data to identify lipid-modified proteins and map modification sites, supporting both targeted studies and proteome-wide discovery.

Technical Principles

Protein lipidation analysis requires specialized enrichment methods due to the hydrophobic nature and low abundance of lipid-modified peptides. MtoZ Biolabs uses selective enrichment strategies to isolate lipidated proteins, followed by high-resolution LC-MS/MS for reliable identification and site localization of lipid-modified residues.

We apply several enrichment techniques to capture lipid-modified peptides:

• Acyl-Biotin Exchange (ABE): Biotinylates palmitoylated proteins for selective capture using streptavidin.
• Resin-Assisted Capture (Acyl-RAC): Thiol-reactive resin isolates S-acylated proteins with high specificity.
• Metabolic Labeling with Alkynyl Fatty Acid Probes: Enables bioorthogonal enrichment of lipid-modified peptides.
• Affinity-based Capture: Selective isolation of lipid-modified proteins using specific reagents.

After enrichment, lipidated peptides are analyzed using high-resolution Orbitrap LC-MS/MS. Fragmentation methods such as HCD and EThcD preserve lipid modifications and enable precise site localization, ensuring accurate detection of lipid-modified residues across a variety of samples.

Protein Lipidation Analysis Service at MtoZ Biolabs

1. Targeted Protein Lipidation Analysis

This service focuses on the detection of lipidation on specific proteins. Purified proteins or immunoprecipitated complexes are analyzed to determine whether they undergo lipidation and to map modification sites. This is ideal for mechanistic studies or for validating candidate proteins involved in lipidation.

2. Lipidation Proteomics

For broader discovery, we offer proteome-wide identification of lipid-modified proteins in complex biological samples such as cell lysates, tissue lysates, or membrane fractions. This service supports research in signaling, metabolism, immune responses, and other pathways where lipidation plays a role.

Figure 1. Workflow of Protein Lipidation Analysis

Why Choose MtoZ Biolabs?

1. Comprehensive Lipidation Profiling

We offer both targeted lipidation analysis and proteome-wide discovery, enabling a wide range of qualitative applications.

2. Advanced Analytical Platforms

High-resolution Orbitrap LC-MS/MS systems combined with lipid-specific enrichment ensure sensitive detection and accurate site identification.

3. High-Confidence Site Localization

HCD and EThcD fragmentation strategies enable precise identification of lipidation sites, distinguishing them from other hydrophobic modifications.

4. Multi-Sample Compatibility

Our platform supports purified proteins, IP samples, whole-cell lysates, tissue lysates, and membrane or organelle fractions.

5. Customized Workflow Design

Workflows are tailored to lipid modification type, sample characteristics, and specific research goals for optimal data relevance and interpretability.

Start Your Project with MtoZ Biolabs

MtoZ Biolabs is committed to providing high-quality Protein Lipidation Analysis Service to support your research into protein modifications and their role in disease mechanisms. Contact our team today to discuss your project needs and plan your customized lipidation analysis strategy.

FAQ

Q1: What types of samples are suitable?

We accept a variety of sample types for lipidation analysis, including purified proteins, immunoprecipitated protein complexes, cell lysates, tissue lysates, and membrane fractions.

Q2: How should I prepare my samples?

• Keep samples fresh or snap-frozen to prevent modification loss
• Use lysis buffers compatible with lipidation workflows, avoiding detergents, high salt, or inhibitors that disrupt enrichment
• Provide sufficient protein input (~100 µg for targeted workflows; ~500 µg for discovery-level profiling)
• For IP samples, ensure adequate purity for clear site localization

For more information, please refer to Sample Submission Guidelines for Proteomics. If you are unsure about sample preparation, our team is available for consultation and can guide you through the process to ensure optimal results.

Q3: What is the service general workflow?

Q4: What data formats are provided?

We provide raw MS data files, processed numerical results in Excel or CSV format, and a comprehensive PDF report that includes detailed analysis of lipid-modified proteins and modification sites.