MtoZ Biolabs provides dedicated Protein Lactylation Analysis Service that supports researchers studying lactate-driven regulatory mechanisms at the protein level. With optimized enrichment workflows and high-resolution LC-MS/MS, we offer qualitative and site-level identification of lactylated peptides from purified proteins, cells, and tissues. Both targeted and discovery-scale options are available to meet the needs of functional studies, mechanistic investigations, and exploratory research.
Overview
Protein lactylation is a lysine modification derived from intracellular lactate and has been observed across many cell types. Studies show that lactylation participates in processes such as immune regulation, cell pluripotency, fibrosis, tumor progression, metabolic adaptation, and neuronal function.
Because lactylation often occurs at low abundance and coexists with other lysine acylations, reliable characterization requires selective enrichment and high-resolution mass spectrometry. Understanding lactylation patterns helps clarify how metabolic changes influence protein behavior and supports research into disease mechanisms and cellular responses.
Lu, Z. et al. Front. Genet. 2024.
Figure 1. Lactylation is Involved in the Regulation of Cellular Physiological and Pathological Processes
Protein Lactylation Analysis Service at MtoZ Biolabs
1. Targeted Protein Lactylation Analysis
MtoZ Biolabs provides targeted lactylation analysis for client-specified proteins. This service focuses on precise identification of lactylated lysine residues using optimized affinity enrichment and high-resolution LC-MS/MS. It is suitable for verification of modification events, site mapping and condition-specific characterization on defined protein targets.
2. Protein Lactylation Proteomics
For broader investigations, MtoZ Biolabs offers high-throughput, proteome-wide lactylation profiling to identify lactylated proteins and modification sites across complex biological samples. This discovery-oriented approach supports studies on metabolic regulation, chromatin remodeling, immune responses and global PTM interactions.
Wu, X. et al. Nat Methods. 2022.
Figure 2. Protein Lactylation Analysis by LC-MS/MS
Why Choose MtoZ Biolabs?
✔ Advanced Analytical Platform
High-resolution Orbitrap LC-MS/MS systems combined with optimized lactylation enrichment protocols ensure sensitive and accurate detection of low-abundance modifications.
✔ Comprehensive Analytical Coverage
We support both targeted analysis for defined proteins and large-scale proteomic studies, enabling flexible experimental designs for diverse research goals.
✔ Broad Sample Compatibility
Our workflows accommodate a wide range of sample types, including cell lysates, tissues, organelles, purified proteins, and immunoprecipitated targets.
✔ Experienced PTM Team
Our scientists have extensive experience in PTM characterization, especially acyl-lysine modifications that require rigorous enrichment, interpretation, and site-level validation.
✔ Customizable Solutions
Services can be tailored to client needs, including target-specific validation, condition-dependent profiling, enriched sample analysis, or exploratory proteomics.
Start Your Project with MtoZ Biolabs
MtoZ Biolabs provides high-quality Protein Lactylation Analysis Services designed to support metabolic research, chromatin studies, and early-stage discovery projects. In addition to lactylation, we offer a full range of post-translational modification analysis services including phosphorylation, ubiquitination, acetylation, and crotonylation. Our integrated workflows and advanced MS platforms ensure accurate and meaningful data tailored to your scientific needs.
Contact us to discuss a customized lactylation analysis plan for your research.
FAQ
Q1: What types of samples are suitable?
We accept a wide range of biological materials, including cell lysates, tissues, organelle fractions, purified proteins, and immunoprecipitated targets. For low-abundance targets, IP-enriched samples can improve detection sensitivity.
Q2: How should I prepare my samples?
Use MS-compatible buffers with minimal detergents and avoid high salt levels. Keep samples frozen at –80 °C and prevent repeated freeze–thaw cycles. For more information, please refer to Sample Submission Guidelines for Proteomics.
If you are unsure about sample preparation, our team is available for consultation and can guide you through the process to ensure optimal results.
Q3: What is the service general workflow?
Q4: What data formats are provided?
Deliverables include Excel results tables, PDF reports with annotated spectra, and optional raw LC-MS/MS files. All data are organized to support downstream verification and publication.
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