MtoZ Biolabs offers advanced MSX-DIA Quantitative Proteomics Services utilizing the cutting-edge multiplexed data-independent acquisition (MSX-DIA) technique. MSX-DIA builds on the traditional DIA method by introducing ion multiplexing technology, improving both quantification accuracy and sample throughput, making it perfect for clinical, biopharmaceutical, and research applications.

What Is MSX-DIA?

MSX-DIA (Multiplexed Data-Independent Acquisition) is an optimized version of the standard DIA (Data-Independent Acquisition) mass spectrometry technique. In traditional DIA, the mass spectrometer captures fragmentation data for ions within predefined mass-to-charge (m/z) windows, but this can lead to interference and reduced accuracy due to co-fragmentation of peptides within those windows. MSX-DIA overcomes this challenge by introducing multiplexing into the data acquisition process. By segmenting the precursor ion selection into multiple, narrower windows and optimizing ion fragmentation, MSX-DIA reduces co-fragmentation interference and allows for more accurate quantification of peptides. This improved methodology increases both sensitivity and throughput, enabling the detection of low-abundance proteins and offering more precise and reliable quantification across complex biological matrices.

Egertson J D. et al. Nature Methods. 2013.

Figure 1. Multiplexed Data-Independent Acquisition with MSX

MSX-DIA Quantitative Proteomics Service at MtoZ Biolabs

MtoZ Biolabs uses high-resolution mass spectrometry to deliver precise, large-scale MSX-DIA quantitative proteomics analysis with improved sensitivity and throughput. Our service is designed to support discovery-driven proteomics, biomarker validation, and disease mechanism studies. Whether you are investigating disease pathways, monitoring therapeutic targets, or analyzing complex proteomes, MSX-DIA provides reliable, reproducible results.

You only need to provide your project objectives and send us your samples. MtoZ Biolabs will handle the entire process, including protein extraction, digestion, MSX-DIA acquisition, data processing, and bioinformatics analysis. We return a comprehensive dataset ready for downstream biological interpretation.

Bioinformatics Analysis

• Data Quality Assessment
• Multivariate PCA Analysis
• Protein Statistical Analysis: Venn Diagram, Volcano Plot
• Functional Annotation: GO Annotation, KEGG Annotation, COG Annotation
• Clustering Analysis: Hierarchical Clustering, K-Means Clustering
• Network Analysis: STRING Analysis

Why Choose MtoZ Biolabs

• Optimized MSX-DIA workflows reduce co-fragmentation and improve quantitative accuracy in complex samples
• High sensitivity and deep proteome coverage, enabling reliable detection of low-abundance proteins for discovery and validation studies
• Flexible experimental design and customizable bioinformatics analysis tailored to your biological questions, target pathways, and downstream validation plans
• Rigorous quality control across sample preparation, MSX-DIA acquisition, and data processing to ensure consistent, high quality datasets
• Professional proteomics and bioinformatics team providing clear reports, publication ready figures, and actionable biological interpretation

Applications of MSX-DIA Quantitative Proteomics Service

MSX-DIA is versatile and can be applied across several research areas:

1. Biomarker Discovery and Clinical Proteomics

Quantify proteins in plasma, serum, CSF, and tissue samples to identify disease biomarkers and monitor disease progression.

2. Biopharmaceutical and Drug Development

Assess protein targets, monitor therapeutic effects, and quantify biomarkers to support drug development and clinical trials.

3. Disease Mechanism Research

Analyze protein expression and signaling pathways to understand disease mechanisms in cancer, neurodegenerative diseases, cardiovascular diseases, and more.

4. Proteomics in Systems Biology

Explore protein interactions, post-translational modifications, and networks in complex biological systems.

FAQ

Q1: What types of samples are suitable?

MSX-DIA Quantitative Proteomics Service supports a broad range of biological samples, including but not limited to:

• Cell pellets from adherent or suspension cultures
• Primary cells isolated from blood, bone marrow, or tissues
• Fresh frozen tissues and small biopsies
• Biofluids such as plasma, serum, CSF, and urine
• Purified protein

If you have other sample types or low-yield materials, we can assess feasibility based on your project goals.

Q2: How should I prepare my samples?

• Snap-freeze cells, tissues, and biofluids where possible and store at -80 °C.
• Avoid repeated freeze-thaw cycles and use low-binding tubes.
• Do not add high levels of strong detergents, salts, or stabilizers.
• Transport samples on dry ice to maintain stability during delivery.

For more information, please refer to Sample Submission Guidelines for Proteomics.

Q3: What is the service general workflow?

Q4: What data formats are provided?

• Raw data files in instrument-specific formats (for example .raw, .wiff, .d)
• Peptide and protein identification and quantification tables in Excel or CSV
• Statistical and bioinformatics outputs, including differential expression and enrichment results, in spreadsheet formats
• Publication-ready visualizations such as heatmaps, volcano plots, PCA and clustering plots in high-resolution PNG or TIFF
• A structured PDF report summarizing workflow, QC metrics, key results, and biological interpretation
• Additional formats or customized deliverables can be provided on request.

Start Your Project with MtoZ Biolabs

Contact us to discuss your experimental design or request a quote. Our technical specialists are available to provide a free business assessment.