MtoZ Biolabs launches the protein mass spectrometry service to provide comprehensive and in-depth identification and analysis of proteins. This service is suitable for protein identification, structural studies, quality control, and functional analysis, offering reliable data support for various life science research and bioproduct development.

Overview

Proteins are core molecules of cellular structure and function, and their complexity arises from diverse sequences, structures, and post-translational modifications. Mass spectrometry uses ionization and mass-to-charge separation principles to identify protein types, sequences, and modification characteristics under high sensitivity and high resolution. Protein analysis based on mass spectrometry can accurately resolve protein composition, expression changes, and structural information, serving as an important approach for studying signaling pathways, disease research, biomarker discovery, and drug target analysis.

Protein Mass Spectrometry Service at MtoZ Biolabs

MtoZ Biolabs uses high-resolution mass spectrometry technology to provide precise identification, structural analysis, and quantitative information of proteins, comprehensively supporting protein characterization for multiple types of samples.

• Identify protein species and sequence information to clarify the protein composition of the sample
• Analyze protein modification status, structural features, and heterogeneity
• Quantitatively compare protein abundance changes under different treatment conditions
• Analyze the molecular weight and peptide features of proteins

Our analysis provides a comprehensive understanding of protein characteristics and biological functions, helping researchers gain deeper insight into protein variation patterns and advance subsequent mechanistic research.

Workflow of Protein Mass Spectrometry Service

1. Sample Pretreatment

Perform desalting, impurity removal, and quantitative evaluation of protein samples, and conduct reduction, alkylation, and enzymatic digestion as needed to ensure the generation of peptides suitable for mass spectrometry analysis.

2. LC Separation

Use high-performance liquid chromatography to separate complex peptide mixtures, reduce matrix interference, and improve the resolution and sensitivity of subsequent mass spectrometry detection.

3. MS/MS Detection

Acquire primary and secondary mass spectrometry data using high-resolution LC-MS/MS to obtain accurate mass information and fragment spectra of peptides.

4. Database Search and Identification

Perform sequence matching based on mass spectrometry data to identify protein IDs, coverage, peptide information, and quantitative results.

5. Result Quality Control and Data Output

Conduct quality evaluation of identification results and generate standardized reports, including identification lists, quantitative information, and related spectra.

Rauniyar, N. et al. Analytical and Bioanalytical Chemistry, 2007.

Figure 1. Workflow of Protein Mass Spectrometry Service.

Why Choose MtoZ Biolabs?

• High-Precision Quantification: Enables highly consistent relative or absolute quantification across multiple sample conditions.
• Wide Dynamic Range Detection: Simultaneously covers high-abundance and trace proteins to achieve comprehensive spectral analysis.
• High Data Reliability: Strict quality control ensures stable and reproducible results.
• Flexible Method Integration: Allows combining different ionization modes and separation strategies according to research needs to increase data depth.
• One-Stop Analytical Capability: Covers the entire workflow from sample preparation and mass spectrometry detection to bioinformatics analysis to improve research efficiency.

Applications of Protein Mass Spectrometry Service

1. Post-Translational Modification Analysis

Supports the detection of multiple types of PTMs such as phosphorylation, acetylation, and glycosylation, and is used to explore protein regulatory mechanisms.

2. Biomarker Discovery

Can screen potential disease-associated proteins in clinical samples, tissues, cells, or body fluids, providing candidate molecules for subsequent validation.

3. Protein Interaction and Pathway Analysis

Combined with Pull-down and Co-IP experiments, clarifies the composition of interacting proteins and assists in mapping signaling pathway networks.

4. Recombinant Protein Expression and Purity Assessment

Used to detect degradation, modifications, or impurities in recombinant proteins, improving the accuracy of quality characterization.

5. Drug Target Study

Can analyze protein alteration patterns after drug treatment and provide data support for mechanism research and drug development.

Deliverables

• Comprehensive Experimental Details
• Materials, Instruments, and Methods
• Protein Identification and Quantification Results
• Spectral and Chromatographic Images
• Bioinformatics Analysis
• Raw Data Files

FAQ

Q1: What types of samples are suitable?

A1: Suitable samples include protein materials with clear origin, good solubility, and basic purity, including purified proteins, cell or tissue extracts, serum or plasma, recombinant proteins, and other samples that can be digested or directly injected. For complex matrices or low-abundance samples, pretreatment can also achieve better identification performance. If you have special sample types, please consult in advance for more accurate recommendations.

Q2: What is the service general workflow?

A2:

Q3: What data formats are provided?

A3: MtoZ Biolabs will provide multiple standardized output formats, including:

• Mass spectrometry raw data files (such as .raw platform formats)
• Processed protein identification or quantification results (Excel/CSV)
• PDF analysis reports (including methods, parameters, identification information, and statistical results)
• Image files (mass spectra, chromatographic peaks, peptide match spectra, etc., in PNG/TIFF formats)

If special analytical requirements exist, data formats can be customized according to project specifications.

Q4: How should I prepare my samples?

A4: To ensure optimal analytical performance, please prepare samples as follows:

• Sample Purity: Avoid components such as high salt, detergents, and glycerol that may inhibit digestion or affect electrospray ionization.
• Sample Storage: Store at 4℃ for short-term use and -80℃ for long-term storage, and avoid repeated freeze-thaw cycles.
• Sample Transport: Use low-temperature conditions (such as ice packs or dry ice) with sealed packaging to prevent leakage or contamination.
• Additional Information: Please provide sample type, concentration, buffer system, pretreatment steps, and research requirements to support the development of a more suitable analytical strategy.

For more information, please refer to Sample Submission Guidelines for Proteomics, Sample Submission Guidelines for Metabolomics.

Start Your Project with MtoZ Biolabs

Contact us to discuss your experimental design or request a quote. Whether you aim to analyze protein composition and modifications or investigate molecular mechanisms and biological functions, MtoZ Biolabs can provide reliable mass spectrometry support for your research.