MtoZ Biolabs offers a specialized Plant Histone Post-Translational Modification Analysis Service to support in-depth characterization of histone modifications in plant samples. This service is designed to deliver reliable identification and quantification of histone post-translational modifications, enabling researchers to investigate epigenetic regulation, chromatin dynamics, and transcriptional control in diverse plant systems.

Overview

Histone post-translational modifications are central regulators of chromatin structure and gene expression in plants. Modifications such as acetylation, methylation, phosphorylation, ubiquitination, and SUMOylation influence nucleosome stability, chromatin accessibility, and the recruitment of regulatory proteins, thereby shaping transcriptional programs involved in development, stress responses, and environmental adaptation.

Comprehensive analysis of plant histone modifications presents unique challenges due to the nuclear localization of histones, their strong association with genomic DNA, and the frequent occurrence of combinatorial modifications on the same histone molecule. Effective histone modification analysis therefore requires controlled isolation of pure histone fractions, sufficient peptide coverage during mass spectrometry analysis, and analytical strategies capable of resolving closely spaced modification sites.

Sharma, M. et al. Proteomes. 2023.

Figure 1. Plant Histone Post-Translational Modifications

Plant Histone Post-Translational Modification Analysis Service at MtoZ Biolabs

MtoZ Biolabs provides an integrated workflow for comprehensive analysis of plant histone post-translational modifications, from histone isolation to site-level identification and quantification.

To ensure high data quality, we have established a dedicated histone isolation and preparation strategy optimized for mass spectrometry analysis. By selectively enriching nuclear fractions and efficiently separating histones from DNA and non-histone components, we obtain highly purified histone proteins suitable for downstream analysis. Chromatographic purification enables separation of individual histone species and variants, while a multi-enzyme digestion approach maximizes peptide coverage and preserves modification information, supporting confident identification and quantitative evaluation of histone PTMs.

Based on this workflow, MtoZ Biolabs supports analysis of a broad range of common plant histone modifications, including but not limited to:

1. Histone Acetylation

Lysine acetylation on histones H3, H4, and variants associated with transcriptional activation and chromatin accessibility.

2. Histone Methylation

Mono-, di-, and trimethylation on lysine and arginine residues involved in epigenetic regulation and gene expression control.

3. Histone Phosphorylation

Phosphorylation on serine, threonine, or tyrosine residues linked to chromatin remodeling, cell cycle regulation, and stress responses.

4. Histone Ubiquitination

Ubiquitin modification on histones related to transcriptional regulation and DNA damage response pathways.

5. Other Histone Acylations

Including propionylation, butyrylation, and related acyl modifications associated with metabolic and regulatory processes in plants.

To meet different experimental objectives, MtoZ Biolabs offers multiple quantitative strategies for plant histone PTM analysis, such as:

• Label-Free Quantitative Analysis for flexible comparison across multiple plant samples or conditions

• Isobaric Labeling-Based Quantification (e.g., TMT) for multiplexed comparison with improved quantitative precision

• Targeted Quantitative Analysis for focused evaluation of specific histone modification sites or patterns

Why Choose MtoZ Biolabs?

1. Specialized Expertise in Plant Histone Analysis

Deep experience with plant chromatin biology and histone modification profiling.

2. High-Confidence Site Localization

Optimized workflows enable accurate discrimination of closely related histone modification types.

3. Comprehensive Modification Coverage

Capability to analyze multiple histone PTMs within a single experimental framework.

4. Flexible Quantitative Options

Support for both discovery-driven profiling and targeted quantitative studies.

5. One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

Start Your Project with MtoZ Biolabs

MtoZ Biolabs provides reliable and scalable solutions for plant histone post-translational modification analysis. Our integrated experimental and analytical workflows support high-confidence epigenetic insights from complex plant samples.

Contact us to discuss your project objectives and initiate your analysis.

FAQ

Q1: What types of samples are suitable?

This service is suitable for a broad range of plant-derived samples, including fresh or frozen plant tissues such as leaves, seedlings, roots, flowers, or seeds. Nuclear-enriched fractions and purified histone preparations are also accepted.

Q2: How should I prepare my samples?

To ensure optimal analytical performance, we recommend the following:

• Collect plant tissues under conditions that minimize protein degradation and modification loss

• Snap-freeze samples in liquid nitrogen immediately after harvesting

• Store samples at low temperature prior to shipment

• Ship samples on dry ice to maintain sample integrity

For more information, please refer to Sample Submission Guidelines for Proteomics. If you are unsure about sample preparation, our team is available for consultation and can guide you through the process to ensure optimal results.

Q3: What is the service general workflow?

Q4: What data formats are provided?

MtoZ Biolabs provides results in multiple standard formats to ensure compatibility with various analysis and visualization tools. Deliverables typically include:

• Raw data files from LC-MS

• Histone modification site identification and quantification tables in Excel or CSV format

• Figures and chromatograms in high-resolution image formats (TIFF or PNG)

• Summary reports in PDF format, including analytical methods, quality control results, and key findings

Additional file formats or data structures can be provided upon request to meet specific research or publication requirements.

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