MtoZ Biolabs provides the 2D-DIGE Quantitative Plant Proteomics Analysis Service based on two-dimensional difference gel electrophoresis combined with multi-channel fluorescence quantification, enabling systematic comparative analysis of protein expression changes across different plant samples or experimental conditions to provide reliable quantitative proteomics data supporting studies on plant physiological regulation, stress responses, and functional protein screening.

The Principle of 2D-DIGE Quantitative Proteomics

2D-DIGE (Two-Dimensional Difference Gel Electrophoresis) is a gel-based quantitative proteomics approach that uses spectrally distinguishable fluorescent dyes to label proteins from different samples. This allows multiple samples to be separated and quantitatively compared within the same two-dimensional gel, effectively reducing inter-gel variability and systematic errors.

In a typical experiment, an internal standard prepared by mixing equal amounts of all samples is introduced and co-run with each experimental group. Proteins are first separated by isoelectric focusing according to their isoelectric points, followed by SDS-PAGE separation based on molecular weight. Multi-channel fluorescence scanning combined with internal-standard-based normalization enables reliable quantification of protein spot abundance and comparison across gels.

Ohlendieck, K. Methods Mol Biol. 2023.

Figure 1. Schematic of 2D-DIGE Analysis

In plant proteomics studies, 2D-DIGE allows direct visualization of protein expression changes as well as migration shifts caused by post-translational modifications or protein isoforms. Protein spots showing significant differences can be excised and further identified by LC-MS/MS.

2D-DIGE Quantitative Plant Proteomics Analysis Service at MtoZ Biolabs

To address the complexity of plant samples and the need for quantitative stability, MtoZ Biolabs has established a standardized 2D-DIGE-based quantitative plant proteomics analysis platform. Key steps, including protein extraction, fluorescent labeling, two-dimensional separation, and image analysis, are performed under unified process control to ensure high comparability and statistical reliability across experimental batches. Our service includes but is not limited to:

• Support for various plant sample types, including leaves, roots, stems, seeds, fruits, and callus tissues
• Comparative analysis of protein expression differences between control and treatment groups
• Optional integration with LC-MS/MS for protein identification and functional interpretation of differential proteins

Why Choose MtoZ Biolabs

• Standardized 2D-DIGE workflows to significantly reduce systematic variation
• Optimized protein extraction and two-dimensional separation strategies for plant samples, minimizing interference from polysaccharides, polyphenols, and pigments
• Internal-standard-based 2D-DIGE quantification combined with strict process control to ensure high reproducibility and comparability
• Integration with high-resolution mass spectrometry platforms (Thermo Fisher Q Exactive HF and Orbitrap Fusion Lumos) for reliable identification and in-depth analysis of differential proteins
• Our pricing is transparent, with no hidden fees or additional costs.

Applications of 2D-DIGE Quantitative Plant Proteomics Analysis Service

• Identification of proteins involved in plant stress responses and stress tolerance mechanisms
• Analysis of protein expression changes during plant growth and development
• Comparative proteomic analysis between different cultivars or mutant lines
• Screening of protein isoforms associated with post-translational modifications
• Preliminary identification of candidate functional proteins related to agronomic traits

Start Your Project with MtoZ Biolabs

If you are conducting quantitative plant proteomics research, you are welcome to contact MtoZ Biolabs. Our technical team will work closely with you to design appropriate analytical strategies based on your research objectives and sample conditions, helping to advance your research efficiently.

FAQs

Q1: What types of samples are suitable?

This service is suitable for total protein samples derived from a wide range of plant tissues and can be applied to comparative analyses across different treatments or plant materials.

Q2: What is the service's general workflow?

Q3: What data formats are provided?

MtoZ Biolabs provides standardized, publication-ready data formats to support downstream analysis, integration, and long-term data archiving. Deliverables typically include:

• Multi-channel 2D-DIGE gel images provided in high-resolution image formats (TIFF or PNG), including individual fluorescence channels and merged images

• Protein spot quantification tables (Excel or CSV format), including normalized spot volumes, fold changes, and statistical analysis results

• Differential protein analysis summary reports (PDF format), outlining experimental workflow, quality control, and key findings

• Mass spectrometry identification results (if LC-MS/MS analysis is included), provided as annotated protein identification tables and supporting spectra summaries

Customized data formats or file structures can be provided upon request to meet specific research or publication requirements.

Q4: How should samples be prepared?

To ensure accuracy, reproducibility, and data quality for the 2D-DIGE Quantitative Plant Proteomics Analysis Service, MtoZ Biolabs recommends preparing and submitting samples according to the following guidelines:

• Sample Type: This service accepts a wide range of plant tissues, including leaves, roots, stems, seeds, fruits, and callus tissues, and supports comparative designs such as control vs. treatment or wild-type vs. mutant analyses.
• Sample Quality: Fresh samples are recommended whenever possible, and visible contamination should be avoided. When feasible, reducing interference from plant-specific components such as polysaccharides, polyphenols, and pigments can improve protein extraction efficiency and gel resolution.
• Sample Amount: Typically, 0.5–1 g of fresh plant tissue or an equivalent amount of frozen material is recommended per sample. Exact requirements may vary depending on tissue type and experimental design.
• Storage and Shipping: Samples should be snap-frozen in liquid nitrogen and stored at −80°C. During shipment, samples should be transported on dry ice in properly sealed containers to preserve protein integrity.
• Sample Documentation: Please provide detailed sample information, including plant species, tissue type, growth conditions, treatment details, biological replicates, and experimental grouping.

For more information, please refer to Sample Submission Guidelines for Proteomics and Sample Submission Guidelines for Metabolomics.